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Popular Articles

Teenagers Show The Government How To Help Tackle Diabetes And Cancer, UK
A group of 15-16 year old students have been reporting directly to the UK government, (Tuesday 30th June), on their proposals for how nanotechnology could be used to help meet the future needs of the healthcare sector.

A New Chapter In The Fight Against Tuberculosis Opened By Unexpected Discovery
A close relative of the microorganism that causes tuberculosis in humans has been found to form spores. This is a sensational finding because researchers have long been convinced that these kinds of bacteria - the mycobacteria - were incapable of forming spores. Leif Kirsebom"s research group at Uppsala University now has photographic proof, obtained while working with the bacteria that causes tuberculosis in fish, to challenge this long-held belief. Their discovery, which has attracted much attention from other scientists, might constitute a new turn in the fight against human tuberculosis.
News of the day
Chembio Awarded Three-Year $3MM NIH SBIR Phase II Grant To Complete DPP(R) Test For Human Leptospirosis
Chembio Diagnostic Systems, Inc. (a wholly-owned subsidiary of Chembio Diagnostics, Inc.) (OTCBB: CEMI) has been awarded a three-year $3 million Small Business Innovative Research (SBIR) Phase II grant from the United States National Institutes of Health (NIH) to fully develop, validate, and commercialize a rapid diagnostic test for leptospirosis for general use worldwide. The test will be developed utilizing Chembio"s patented Dual Path Platform (DPP®) technology together with proprietary reagents developed by Cornell University and the Oswaldo Cruz Foundation at the Brazilian Ministry of Health.
Public Health

ChIP-Seq, Drosophila Targeted Mutagenesis Featured In Cold Spring Harbor Protocols

High-throughput whole-genome analysis is becoming a standard laboratory approach for investigating cellular processes. Next-generation sequencing is replacing microarrays as the technique of choice for genome-scale analysis, because it offers advantages in both sensitivity and scale. The June issue of Cold Spring Harbor Protocols features "Native Chromatin Preparation and Illumina/Solexa Library Construction" from Keji Zhao and colleagues at the National Heart, Lung and Blood Institute. The article describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using native chromatin and the Solexa/Illumina Genome Analyzer. Step-by-step instructions are given for purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP) and construction of a library for sequencing. The article is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/6/pdb.prot5237). Mutational analysis has long been a valuable tool for deciphering gene function. However, systematic repeated targeting of a single locus is difficult and is not a routine approach in multicellular organisms. Yikang Rong and colleagues at the National Cancer Institute have developed the Site-specific Integrase mediated Repeated Targeting (SIRT) method to facilitate targeted mutagenesis in Drosophila melanogaster. SIRT targets a landing site for the phage phiC31 integrase and allows the generation of several genetic variants at a locus of interest without having to perform multiple experiments. SIRT requires the construction of a series of plasmid vectors with varying arrangements of DNA elements. By taking advantage of bacterial recombineering approaches, SIRT bypasses the shortcomings of traditional cloning techniques that rely on the availability of convenient restriction enzyme cut sites. This method, "SIRT Combines Homologous Recombination, Site-Specific Integration, and Bacterial Recombineering for Targeted Mutagenesis in Drosophila," is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/6/pdb.prot5236). David Crotty Cold Spring Harbor Laboratory


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